Molecular Entomology
§ Senior Research Fellowship (2019–2022): Council of Scientific and Industrial Research (CSIR), New Delhi, India.
§ Junior Research Fellow (2017–2019): Council of Scientific and Industrial Research (CSIR), New Delhi, India.
§ Qualified CSIR-NET, June 2015- conducted by CSIR, New Delhi, India.
“Identification and characterization of putative interacting protein partners of Dengue Virus envelope(E) protein in the insect vector Aedes aegypti.”
Technical Proficiency
§ Molecular analysis: Isolation and estimation of genomic DNA/RNA, cDNA synthesis, PCR amplification, Restriction digestion, carried out agarose and polyacrylamide gel electrophoresis immunoblot analysis of different protein.
§ Insect cell culture: C6/36 cell line maintenance and Dengue virus propagation, isolation and storage. SF9/SF21 cell line maintenance, propagation for recombinant protein production.
§ Animal handling: BALB/c inbred mice
§ Aedes aegypti colony maintenance and propagation
§ Protein-protein interaction: Yeast two-hybrid
Phage display library screening
Pull-down assay/reverse pull down
Immunoprecipitation
Coimmunoprecipitation
Gene Expression (qualitative and quantitative analysis)
§Antibody inhibition assay using dsRNA and polyclonal antibodies (in insect Aedes aegypti.)
§ Confocal microscopy immunofluorescence
§ Microbial techniques: Plasmid isolation from bacteria, cloning and transformation.
§ Recombinant protein production and purification in heterologous expression system
§ Proteomics: Isolation and estimation of proteins, SDS-PAGE, Western blotting.
§ Bioinformatics and computer skills: Knowledge of basic computational bioinformatics software (for primer designing, protein modelling, BLAST, other web-based bioinformatics tools).
§Protein-protein Docking.
§ Graphpad prism and R Statistical Tools